mouse anti smarcal1 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
mouse anti smarcal1
Mouse Anti Smarcal1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+smarcal1/SmarcAL1+Antibody/pm41866377-381-5-9
Average 94 stars, based on 63 article reviews
Mouse Anti Smarcal1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+smarcal1/SmarcAL1+Antibody/pm41866377-381-5-9
Average 94 stars, based on 63 article reviews
mouse anti smarcal1 - by Bioz Stars,
2026-10
94/100 stars
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Blocking Assay:Article Title: Stress-triggered hematopoietic stem cell proliferation relies on PrimPol-mediated repriming. Article Snippet: Cells were pre-fixated in 0.1% formaldehyde (Sigma), pre-extracted using CSK-buffer (10mM HEPES pH = 7.4, 0.1M NaCl, 0.3M sucrose, 3mMMgCl2) for 5 min, fixed for 15 min with 4% formaldehyde (Sigma) and post-fixated usingmethanol for 20min at 20 C. After blocking with 5%BSA +10% FBS blocking solution, cells were incubated with Click-iT Cell reaction buffer using azide-Biotin (Vanderbilt University). .. Afterward, primary antibodies were applied for 1h in blocking solution (see Immunostaining) with either rabbit anti-PrimPol (1:500) – produced in R. Freire’s lab immunizing a rabbit with a peptide containing amino acids 1- 240 from murine primpol – or with Immunostaining:Article Title: Stress-triggered hematopoietic stem cell proliferation relies on PrimPol-mediated repriming. Article Snippet: Cells were pre-fixated in 0.1% formaldehyde (Sigma), pre-extracted using CSK-buffer (10mM HEPES pH = 7.4, 0.1M NaCl, 0.3M sucrose, 3mMMgCl2) for 5 min, fixed for 15 min with 4% formaldehyde (Sigma) and post-fixated usingmethanol for 20min at 20 C. After blocking with 5%BSA +10% FBS blocking solution, cells were incubated with Click-iT Cell reaction buffer using azide-Biotin (Vanderbilt University). .. Afterward, primary antibodies were applied for 1h in blocking solution (see Immunostaining) with either rabbit anti-PrimPol (1:500) – produced in R. Freire’s lab immunizing a rabbit with a peptide containing amino acids 1- 240 from murine primpol – or with Produced:Article Title: Stress-triggered hematopoietic stem cell proliferation relies on PrimPol-mediated repriming. Article Snippet: Cells were pre-fixated in 0.1% formaldehyde (Sigma), pre-extracted using CSK-buffer (10mM HEPES pH = 7.4, 0.1M NaCl, 0.3M sucrose, 3mMMgCl2) for 5 min, fixed for 15 min with 4% formaldehyde (Sigma) and post-fixated usingmethanol for 20min at 20 C. After blocking with 5%BSA +10% FBS blocking solution, cells were incubated with Click-iT Cell reaction buffer using azide-Biotin (Vanderbilt University). .. Afterward, primary antibodies were applied for 1h in blocking solution (see Immunostaining) with either rabbit anti-PrimPol (1:500) – produced in R. Freire’s lab immunizing a rabbit with a peptide containing amino acids 1- 240 from murine primpol – or with Western Blot:Article Title: Strand annealing and motor driven activities of SMARCAL1 and ZRANB3 are stimulated by RAD51 and the paralog complex Article Snippet: .. The antibodies used for Western blotting, immunoprecipitation and DNA fiber assay were used as follows: Mouse anti-RAD51B (Santa Cruz sc-377192; 1:1000 dilution for WB), Mouse anti-XRCC2 (Santa Cruz sc-365854; 1:1000 dilution for WB), Rabbit anti-RAD51C (Abcam ab95069; 1:1000 dilution for WB), Rabbit anti-RAD51D (Abcam ab202063; 1:1000 dilution for WB), Rat anti-BrdU (Abcam ab6326; 1:100 dilution for DNA fiber assay), Mouse anti-BrdU (Becton Dickinson BD347580; 1:100 dilution for DNA fiber assay), Article Title: RF-SIRF reveals a replication stress-specific epigenetic code by spatio-temporal mapping of reversed forks. Article Snippet: Antibodies used for SIRF assays are as follows: mouse anti-biotin (BN-34, 1:100; Sigma-Aldrich), rabbit anti-biotin (D5A7, 1:200; Cell Signaling Technology), mouse anti-dsDNA (MAB1293, 1:100; Millipore), mouse antidsDNA (CBL186, 1:100; Millipore), rabbit anti- GFP (2555S, 1:200; Cell signaling technology), rabbit anti-H3K9me3 (ab8898, 1:500; Abcam), mouse anti-ssDNA (clone 16-19, 1:100; Millipore), mouse anti- Lamin A/C (4C11, 1:50; Cell signaling technology) rabbit anti-H3K4me3 (ab8580, 1:500; Abcam), rabbit anti-H4K16ac (ab109463, 1:200; Abcam), mouse anti-BrdU (B-44, 1:100; BD347580), rabbit anti-BrdU (1:400; GTX128091), mouse anti-DNAPKcs (1:100; sc-390849), rabbit anti-histone H3 (1:200; ab1791), mouse anti-H3K4me3 (1:100; Sigma Aldrich 05-1339), mouse anti-MRE11 (1:200, ab214). .. Antibodies used for immunoblotting are Article Title: Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair. Article Snippet: For immunofluorescence studies, the following antibodies were used: Rat monoclonal anti-BrdU 1:100 (Abcam ab6326); Mouse anti-BrdU BD Biosciences 1:10 (347580); Alexa FluorTM 488 (Invitrogen A10266) or Alexa FluorTM 594 (Invitrogen A10270); (Goat anti-Rat Cy5 2:25 (Abcam ab6565), Goat anti-mouse Cy3 2:25 (Abcam ab97035); Rabbit anti-phospho histone H3 Ser 10 (Cell Signaling Technology 9701S) 1:200, Rabbit anti-RAD51 (Abcam ab133534); Rabbit anti-MRE11 (Cell Signaling Technology 4895S), Mouse anti-Biotin (Jackson Immuno 200-002-211); Duolink PLA Red (Sigma Aldrich DUO92101); Mouse anti-ssDNA (DSHB A UT OANTI-SSDNA-S); Goat anti-Mouse BV480 (Jackson 115-685-166); Invitrogen Goat anti-mouse IgG AF488 1:175 (A-11001); Invitrogen Goat anti-Rat IgG AF594 1:350 (A11007). .. For immunoblotting, the following antibodies were used: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Millipore OP95100UG) 1:500; Mouse anti-MSH2 (Santa Cruz sc-376384) 1:1000; Rabbit anti-MLH1 (Abcam ab92312) 1:1000; Rabbit anti-DCAF14 (Novus Biologicals NBP2-33883) 1:500; Rabbit anti-KU70 (Abcam ab92450) 1:7000; Mouse anti-cyclin A (B-8) (Santa Cruz sc-271682) 1:1000; Rabbit anti-cyclin B1 (Cell Signaling Technologies 4138S) 1:1000; Mouse anticyclin E1 (Cell Signaling Tech 4129T) 1:1000; Mouse antiPCNA (Santa Cruz sc-56) 1:1000; Article Title: RF-SIRF defining reversed DNA replication forks with single-cell and spatio-temporal resolution reveals a replication stress specific epigenetic code Article Snippet: Antibodies used for SIRF assays are as follows: mouse anti-biotin (Sigma-Aldrich), rabbit anti-biotin (Cell Signaling Technology), mouse anti-dsDNA (Millipore), mouse anti-dsDNA (Millipore), rabbit anti-GFP (Cell signaling technology), rabbit anti-H3K9me3 (Abcam), mouse anti-ssDNA (Millipore), mouse anti-Lamin A/C (Cell signaling technology) rabbit anti-H3K4me3 (Abcam), rabbit anti-H4K16ac (Abcam). .. Antibodies used for immunoblotting are Article Title: Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair Article Snippet: For immunofluorescence studies, the following antibodies were used: Rat monoclonal anti-BrdU 1:100 (Abcam ab6326); Mouse anti-BrdU BD Biosciences 1:10 (347580); Alexa Fluor 488 (Invitrogen A10266) or Alexa Fluor 594 (Invitrogen A10270); (Goat anti-Rat Cy5 2:25 (Abcam ab6565), Goat anti-mouse Cy3 2:25 (Abcam ab97035); Rabbit anti-phospho histone H3 Ser 10 (Cell Signaling Technology 9701S) 1:200, Rabbit anti-RAD51 (Abcam ab133534); Rabbit anti-MRE11 (Cell Signaling Technology 4895S), Mouse anti-Biotin (Jackson Immuno 200-002-211); Duolink PLA Red (Sigma Aldrich DUO92101); Mouse anti-ssDNA (DSHB AUTOANTI-SSDNA-S); Goat anti-Mouse BV480 (Jackson 115-685-166); Invitrogen Goat anti-mouse IgG AF488 1:175 (A-11001); Invitrogen Goat anti-Rat IgG AF594 1:350 (A-11007). .. For immunoblotting, the following antibodies were used: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Millipore OP95-100UG) 1:500; Mouse anti-MSH2 (Santa Cruz sc-376384) 1:1000; Rabbit anti-MLH1 (Abcam ab92312) 1:1000; Rabbit anti-DCAF14 (Novus Biologicals NBP2-33883) 1:500; Rabbit anti-KU70 (Abcam ab92450) 1:7000; Mouse anti-cyclin A (B-8) (Santa Cruz sc-271682) 1:1000; Rabbit anti-cyclin B1 (Cell Signaling Technologies 4138S) 1:1000; Mouse anti-cyclin E1 (Cell Signaling Tech 4129T) 1:1000; Mouse anti-PCNA (Santa Cruz sc-56) 1:1000; Immunoprecipitation:Article Title: Strand annealing and motor driven activities of SMARCAL1 and ZRANB3 are stimulated by RAD51 and the paralog complex Article Snippet: .. The antibodies used for Western blotting, immunoprecipitation and DNA fiber assay were used as follows: Mouse anti-RAD51B (Santa Cruz sc-377192; 1:1000 dilution for WB), Mouse anti-XRCC2 (Santa Cruz sc-365854; 1:1000 dilution for WB), Rabbit anti-RAD51C (Abcam ab95069; 1:1000 dilution for WB), Rabbit anti-RAD51D (Abcam ab202063; 1:1000 dilution for WB), Rat anti-BrdU (Abcam ab6326; 1:100 dilution for DNA fiber assay), Mouse anti-BrdU (Becton Dickinson BD347580; 1:100 dilution for DNA fiber assay), Incubation:Article Title: MDC1 counteracts replication fork reversal and mediates chemosensitivity in BRCA1/2-deficient tumors. Article Snippet: Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45 μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris, pH 7.5, 0.9% NaCl, 0.05% Tween-20). .. Membranes were incubated with the following primary antibodies: primary mouse monoclonal anti-MDC1 antibody (1:1000 #M2444, Sigma Aldrich), Vinculin (E1E9V) XP® Rabbit mAb (1:1000, #13901, Cell Signaling), rabbit polyclonal anti-RPA32 (1:1000, #A300-244A, Bethyl Laboratories), rabbit recombinant monoclonal anti-phospho RPA32 (S4/S8) (1:1000, #A700-009, Bethyl Laboratories), rabbit polyclonal anti-CHK1 (1:5000, #NB100-464, Novus Biologicals), rabbit monoclonal anti-phospho CHK1 (S345) (133D3) (1:1000, #2348, Cell Signaling), rabbit polyclonal anti-KAP1 (1:1000, #NB500-158, Novus Biologicals), rabbit polyclonal anti-phospho KAP1 (S824) (1:1000, #A300-767A, Bethyl Laboratories), rabbit anti-MRE11 (1:5000, NB100-142, Novus), Article Title: MDC1 counteracts replication fork reversal and mediates chemosensitivity in BRCA1/2-deficient tumors Article Snippet: Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45 μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100 mM Tris, pH 7.5, 0.9% NaCl, 0.05% Tween-20). .. Membranes were incubated with the following primary antibodies: primary mouse monoclonal anti-MDC1 antibody (1:1000 #M2444, Sigma Aldrich), Vinculin (E1E9V) XP® Rabbit mAb (1:1000, #13901, Cell Signaling), rabbit polyclonal anti-RPA32 (1:1000, #A300-244A, Bethyl Laboratories), rabbit recombinant monoclonal anti-phospho RPA32 (S4/S8) (1:1000, #A700-009, Bethyl Laboratories), rabbit polyclonal anti-CHK1 (1:5000, #NB100-464, Novus Biologicals), rabbit monoclonal anti-phospho CHK1 (S345) (133D3) (1:1000, #2348, Cell Signaling), rabbit polyclonal anti-KAP1 (1:1000, #NB500-158, Novus Biologicals), rabbit polyclonal anti-phospho KAP1 (S824) (1:1000, #A300-767A, Bethyl Laboratories), rabbit anti-MRE11 (1:5000, NB100-142, Novus), Recombinant:Article Title: MDC1 counteracts replication fork reversal and mediates chemosensitivity in BRCA1/2-deficient tumors. Article Snippet: Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45 μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris, pH 7.5, 0.9% NaCl, 0.05% Tween-20). .. Membranes were incubated with the following primary antibodies: primary mouse monoclonal anti-MDC1 antibody (1:1000 #M2444, Sigma Aldrich), Vinculin (E1E9V) XP® Rabbit mAb (1:1000, #13901, Cell Signaling), rabbit polyclonal anti-RPA32 (1:1000, #A300-244A, Bethyl Laboratories), rabbit recombinant monoclonal anti-phospho RPA32 (S4/S8) (1:1000, #A700-009, Bethyl Laboratories), rabbit polyclonal anti-CHK1 (1:5000, #NB100-464, Novus Biologicals), rabbit monoclonal anti-phospho CHK1 (S345) (133D3) (1:1000, #2348, Cell Signaling), rabbit polyclonal anti-KAP1 (1:1000, #NB500-158, Novus Biologicals), rabbit polyclonal anti-phospho KAP1 (S824) (1:1000, #A300-767A, Bethyl Laboratories), rabbit anti-MRE11 (1:5000, NB100-142, Novus), Article Title: MDC1 counteracts replication fork reversal and mediates chemosensitivity in BRCA1/2-deficient tumors Article Snippet: Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45 μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100 mM Tris, pH 7.5, 0.9% NaCl, 0.05% Tween-20). .. Membranes were incubated with the following primary antibodies: primary mouse monoclonal anti-MDC1 antibody (1:1000 #M2444, Sigma Aldrich), Vinculin (E1E9V) XP® Rabbit mAb (1:1000, #13901, Cell Signaling), rabbit polyclonal anti-RPA32 (1:1000, #A300-244A, Bethyl Laboratories), rabbit recombinant monoclonal anti-phospho RPA32 (S4/S8) (1:1000, #A700-009, Bethyl Laboratories), rabbit polyclonal anti-CHK1 (1:5000, #NB100-464, Novus Biologicals), rabbit monoclonal anti-phospho CHK1 (S345) (133D3) (1:1000, #2348, Cell Signaling), rabbit polyclonal anti-KAP1 (1:1000, #NB500-158, Novus Biologicals), rabbit polyclonal anti-phospho KAP1 (S824) (1:1000, #A300-767A, Bethyl Laboratories), rabbit anti-MRE11 (1:5000, NB100-142, Novus), Cell-Signaling:Article Title: Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair Article Snippet: For immunofluorescence studies, the following antibodies were used: Rat monoclonal anti-BrdU 1:100 (Abcam ab6326); Mouse anti-BrdU BD Biosciences 1:10 (347580); Alexa Fluor 488 (Invitrogen A10266) or Alexa Fluor 594 (Invitrogen A10270); (Goat anti-Rat Cy5 2:25 (Abcam ab6565), Goat anti-mouse Cy3 2:25 (Abcam ab97035); Rabbit anti-phospho histone H3 Ser 10 (Cell Signaling Technology 9701S) 1:200, Rabbit anti-RAD51 (Abcam ab133534); Rabbit anti-MRE11 (Cell Signaling Technology 4895S), Mouse anti-Biotin (Jackson Immuno 200-002-211); Duolink PLA Red (Sigma Aldrich DUO92101); Mouse anti-ssDNA (DSHB AUTOANTI-SSDNA-S); Goat anti-Mouse BV480 (Jackson 115-685-166); Invitrogen Goat anti-mouse IgG AF488 1:175 (A-11001); Invitrogen Goat anti-Rat IgG AF594 1:350 (A-11007). .. For immunoblotting, the following antibodies were used: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Millipore OP95-100UG) 1:500; Mouse anti-MSH2 (Santa Cruz sc-376384) 1:1000; Rabbit anti-MLH1 (Abcam ab92312) 1:1000; Rabbit anti-DCAF14 (Novus Biologicals NBP2-33883) 1:500; Rabbit anti-KU70 (Abcam ab92450) 1:7000; Mouse anti-cyclin A (B-8) (Santa Cruz sc-271682) 1:1000; Rabbit anti-cyclin B1 (Cell Signaling Technologies 4138S) 1:1000; Mouse anti-cyclin E1 (Cell Signaling Tech 4129T) 1:1000; Mouse anti-PCNA (Santa Cruz sc-56) 1:1000; |